Deoxyribonuclease from Salmon Testes

Deoxyribonuclease from Salmon Testes

A procedure is described for the purification of salmon testis deoxyribonuclease II by means of acid extraction, fractional precipitation with ammonium sulfate, heat denaturation of extraneous proteins, and ethanol fractionation. This process separates the deoxyribonuclease activity from that of ribonuclease, phosphatase, phosphodiesterase, and protease. Over 50 per cent of the activity is reta...

Deoxyribonuclease Activity

The relationship of catalase, cystinase, and deoxyribonuclease activity to toxinogeny in Corynebacterium diphtheriae was examined. Mutants deficient in each activity were isolated after mutagenization of strain C4 with nitrosoguanidine. All mutants were converted to toxinogeny after lysogenization with beta-converting phage, thus establishing that there is no absolute link between toxin product...

A novel deoxyribonuclease inhibitor from Micromonospora.

Deoxyribonuclease activity of micrococci from clinical sources.

from Chum Salmon

Cystatin, a cysteine proteinase inhibitor, was isolated from chum salmon (Oncorilynch"s keta) pituitary glands by ion-exchange chromatography on Mono-Q, gel filtration on Superdex 75, and reverse-phase HPLC on an ODS fo1]owing ethanol-ammonium acetate extraction. Salmon pituitary cystatin was equipotent te chicken egg-white cystatin in the papain inhibitory assay. The cystatin consists of 111 a...